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New method aids study of poultry infections

Chloe RyanBy Chloe RyanFebruary 18, 20202 Mins Read
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Fluorescent green immune cells in chicken lung tissue are seen to target models of infection, coloured red
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A novel way to study key poultry infections and aid the development of vaccines has been developed by researchers.
Their approach can be used to study the respiratory tract in poultry, which is an important entry route for disease and delivery method for vaccines. It potentially reduces the number of animals required for scientific studies. Sampled cross-sections of lung tissue from adult chickens can mimic the whole lung in a live bird, scientists found.
Samples remained viable for testing over several weeks, enabling researchers to track their response to treatments over time.
Researchers from the Roslin Institute and the French National Research Institute for Agriculture, Food and Environment (INRAE) studied adult chicken lung samples recovered using a method known as precision-cut lung slicing.
The approach can generate tens to hundreds of thin samples from a single organ. The team found that immune cells in the tissue were suitable for testing their response to vaccines or disease treatment.
They also worked out the optimum culture conditions in which to preserve the shape and mixed populations of cells in samples.
“These samples can be applied to a range of research and are especially useful to investigate respiratory infections. We are currently using these samples to understand flu, help us find new treatments and understand why some chickens can cope with viruses while other chickens cannot,” said Dr Karen Bryson from the Roslin Institute.
“Working in close collaboration with our European colleagues has enabled us to develop this useful technique, which will support our understanding of poultry health while limiting the use of animals in research,” said Professor Lonneke Vervelde from the Roslin Institute
Their study, published in Veterinary Research, was supported by the European Union’s Horizon 2020 Research and Innovation Programme, along with a collaboration between the Biotechnology and Biological Sciences Research Council and the US department of department of Agriculture (USDA).

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Chloe Ryan

Editor of Poultry Business, Chloe has spent the past decade writing about the food industry from farming, through manufacturing, retail and foodservice. When not working, dog walking and reading biographies are her favourite hobbies.

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